HA006 - Hatton
Área: 4 - Ortodontia

Maxillary dentoskeletal outcomes of the expander with the differential opening and the fan-type expander: a randomized clinical trial
Massaro C, Ruellas ACO, Cevidanes LHS, Yatabe MS, Janson G, Miranda F, Lauris JRP, Garib D
Odontopediatria, Ortodon - UNIVERSIDADE DE SÃO PAULO - BAURU
Conflito de interesse: Autodeclarado "Patente referente ao expansor com abertura diferencial no nome da autora Daniela Gamba Garib (PI 1101050-9, Instituto nacional de patente e indústria-Brasil)."

The aim of this randomized clinical trial was to compare the maxillary expander with differential opening (EDO) and the fan-type expander (FE) in the mixed dentition by means of cone-beam computed tomography (CBCT) three-dimensional models superimposed on the cranial base. Forty-eight patients with maxillary dental arch constriction were randomly allocated into 2 study groups. Group EDO was composed of 24 patients (mean age of 7.62 years) treated with the EDO. Group FE comprised 24 patients (mean age of 7.83 years) treated with the FE. CBCT scans were acquired before and after rapid maxillary expansion. Three-dimensional dentoskeletal changes were assessed after cranial base superimposition using the software ITK-SNAP and 3D Slicer. T or Mann-Whitney U tests with Holm-Bonferroni correction were used for intergroup comparisons (P<0.05). Maxillary lateral displacements were greater in the EDO group, while the vertical and anteroposterior changes were similar in both groups. The increase in the intercanine distance and the canine buccal inclination were slightly greater in the FE group, while the intermolar changes and the molar buccal inclination were greater in the EDO group.
The expander with differential opening (EDO) produced a greater transversal skeletal expansion compared to the fan-type expander (FE), with similar vertical and anteroposterior effects. Dental changes were greater in the molar region for patients treated with EDO and in the canine region for patients treated with FE.
(Apoio: CAPES  |  FAPs - FAPESP  N° 2017/12911-9  |  NIDCR R01 DE024450)

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HA007 - Hatton
Área: 5 - Materiais Dentários

Nanoscale biomimetic mineralization of bioprinted gelatin methacryloyl for tissue engineering
Balbinot GS, Subbiah R, Athirasala A, Collares FM, Bertassoni LEB
Odontologia Conservadora - UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL
Conflito de interesse: Não há conflito de interesse

Mimicking the complex structure of bone tissue is key for effective bone regeneration. This study aimed to develop a unique process to 3D bioprint microscale cell-laden scaffolds that mimick the bone nanoscale structure and function with precision and can be delivered via injection for minimally invasive regenerative procedures. Gelatin methacryloyl (GelMA) hydrogels were produced by stereolithography and used as an organic substrate for biomimetic nanoscale mineralization via a proprietary protein-mediated biomimetic mineralization process with osteopontin in a Ca²⁺ and PO₄^3- supersaturated media. Mineralization was optimized for different media, gelMA concentrations, and cross-linking times. The mineral content and mechanical properties were evaluated, and optimized hydrogels were used for dental pulp stem cells (DPSCs) encapsulation. The printability was tested for injectable mineralized microgels production. After the screening, 10wt% gelMA printed for 25s and mineralized in α-MEM was chosen, reaching an elastic modulus of 123.94kPa. Mineralized hydrogels DPSCs viability and differentiation. Injectable flower-shaped microgels (900µm) were successfully printed and mineralized.
The nanoscale mineralization of bone-like cell-laden bioprinted microgels was demonstrated for the first time. The obtained nanostructure evidence the ability of these materials to mimic the intra- and extracellular environments of bone. Mineralized microgels were successfully produced for injectable minimally invasive regenerative procedures.

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