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FC028 - Fórum Científico
Área: 9 - Ciências do comportamento / Saúde Coletiva
Apresentação: 11/09 (Sábado) - Horário: 14h00 - 15h30 - Sala: 6
Versão brasileira do Positive Oral Health and Well-Being (B-POHW): avaliando a saúde bucal positiva
Perazzo MF, Ortiz FR, Zini A, Büssing A, Vered Y, Martins-Júnior PA, Granville-Garcia AF, Paiva SM
Saúde Coletiva - UNIVERSIDADE FEDERAL DE GOIÁS
Conflito de interesse: Não há conflito de interesse
O presente estudo objetivou adaptar transculturalmente e testar as propriedades psicométricas da versão brasileira do Positive Oral Health and Well-Being (B-POHW). Após a tradução para o português brasileiro, a adaptação transcultural do B-POHW foi pré-testada, seguida de coleta de dados para a testagem das propriedades psicométricas. O ajuste do modelo foi testado em 209 participantes (média de idade: 39,36, +12,26) por meio da Análise Fatorial Confirmatória com indicadores categóricos em modelos bifatorial e de estrutura simples. Medidas externas de validação foram representadas por questionários referentes a dados sociodemográficos, hábitos e percepção sobre a saúde bucal, e bem-estar geral. A experiência de cárie dentária foi diagnosticada clinicamente pelos critérios da Organização Mundial da Saúde (índice CPO-D). Para confiabilidade teste-reteste, o B-POHW foi reaplicado em 53 participantes, quinze dias depois. Os seguintes resultados foram encontrados: a) o modelo bifatorial apresentou o melhor ajuste; b) satisfatória consistência interna (α de Cronbach e ω de McDonald> 0,8); c) forte correlação de Spearman para o Fator Global no teste-reteste (rs = 0,85); d) representação do construto de acordo com a base teórica da saúde bucal positiva.
O B-POHW é psicometricamente adequado para ser usado no contexto brasileiro e as evidências de sua estrutura interna confirmaram sua base teórica para mensurar a saúde bucal positiva. Esses achados avançam nas abordagens holísticas, permitindo avaliar a saúde bucal positiva na prática odontológica no Brasil.
(Apoio: CNPq N° 205043/2018-6 | CAPES N° 001)
HA003 - Hatton
Área: 3 - Controle de infecção / Microbiologia / Imunologia
Apresentação: 10/09 (Sexta-feira) - Horário: 08h30 - 10h00 - Sala: 7
Effects of nano-sized sodium hexametaphosphate and fluoride on dual-species biofilms of Streptococcus mutans and Candida albicans
Sampaio C, Delbem ACB, Fernandes AVP, Hosida TY, Morais LA, Camargo ER, Monteiro DR, Pessan JP
Odontologia Infantil e Social - UNIVERSIDADE ESTADUAL PAULISTA - ARAÇATUBA
Conflito de interesse: Não há conflito de interesse
This study evaluated the effects of nano-sized sodium hexametaphosphate (HMPnano), combined or not with fluoride (F), on dual-species biofilms of Streptococcus mutans and Candida albicans. Solutions containing micrometric HMP (HMPmicro) or HMPnano were prepared at 0.5 or 1%, combined or not with 1,100 ppm F; 1,100 ppm F and artificial saliva were tested as positive and negative controls, respectively. Dual-species biofilms of S. mutans and C. albicans, grown in microtiter plates, were treated (1 min) with the solutions at 72, 78 and 96 h from the beginning of their formation. Biofilms were analyzed by colony-forming unit counting (CFU), metabolic activity (XTT assay), and production of total biomass (crystal violet assay). Data were submitted to ANOVA or Kruskal Wallis test, followed by Tukey's or Student-Newman-Keuls' tests (p<0.05). HMPnano at 1% + F led to the highest CFU reduction of S. mutans, followed by HMP micro at 1% + F and positive control (similar to each other), and the remaining groups; CFU counts of C. albicans were not affected by any solution assessed. Furthermore, HMPnano at 1% led to significant lower metabolic activity compared to all other groups (except for HMPnano at 1% + F). Also, all test solutions promoted significant reductions in biofilm biomass compared to both positive and negative controls.
It can be concluded that HMPnano promoted higher antibiofilm effects compared with its micrometric counterpart for most of the variables assessed, besides having a synergist action with F on CFU reduction of S. mutans.
(Apoio: CAPES N° 001 | CAPES N° 88881.068437/2014-01 | CNPq N° 123611/2019-9)
HA005 - Hatton
Área: 3 - Fisiologia / Bioquimica / Farmacologia
Apresentação: 10/09 (Sexta-feira) - Horário: 08h30 - 10h00 - Sala: 7
Metabolomic identification of novel salivary biomarkers for diagnostic and monitoring Diabetes
Moura DV, Caixeta DC, Santos P, Martins MM, Goulart LR, Sabino-Silva R
Fisiologia - UNIVERSIDADE FEDERAL DE UBERLÂNDIA
Conflito de interesse: Não há conflito de interesse
The diagnostic and monitoring of glycemia is an invasive and painful in diabetes mellitus (DM).Consequently, the search for non-invasive diagnostic biomarkers is of great interest in DM. The limitation to use saliva in clinical settings can be related to the reduced chemical composition knowledge. Here, a characterization of the salivary metabolome is presented in a diabetic animal model. Fluid chromatography coupled to mass spectrometry with time-of-flight system were employed to spot the metabolites in rats saliva. Fifteen Wistar rats were divided in non-diabetic (ND), diabetic (D) and diabetic 6U-treated of insulin (D6U). DM was induced by an intraperitoneal injection (60 mg/kg) of streptozotocin (STZ). The animals were submitted to 28 days of diabetes, and on the 21st day, the insulin- or placebo-treatment was started (#CEUA 13/16). The glycemia and urinary glucose confirmed the diabetic state. The metabolic salivary profile identified hundreds of novel compounds with potential to discriminate diabetes. Principal Component Analysis and clustering indicates 3 main candidates as novel salivary metabolites for salivary screening of diabetes: N-Arachidonoyl tyrosine, 1-11-Eicosenoyl-Triacylglycerol and Dimethylphosphatidylethanolamine (22:5(7Z/24:1(15Z) HMDB, (ANOVA).
Altogether, this salivary metabolomic analysis indicates novel salivary metabolites candidates to be applied in salivary diagnostic salivary platforms for non-invasive diabetic diagnostic platforms and for the salivary monitoring tool during insulin treatment.
(Apoio: CAPES N° (#23038.014934/2020-59). | FAPEMIG N° (#APQ-02872-16) | INCT-TeraNano N° (465669/2014-0))
HA006 - Hatton
Área: 4 - Odontopediatria
Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7
Selective Outcome Reporting (SOR) in Pediatric Dentistry restorative treatment Randomized Clinical Trials - A systematic Review
Elagami RA, Tedesco TK, Pannuti CM, Seabra G, Braga MM, Mendes FM, Raggio DP
Odontopediatria e Ortodontia - UNIVERSIDADE DE SÃO PAULO - SÃO PAULO
Conflito de interesse: Não há conflito de interesse
We aimed to evaluate the prevalence of SOR in the randomized clinical trials focusing on restorative treatment in primary molars. We searched for RCTs protocol registries in the ClinicalTrials.gov and WHO platform up to April/2021. We included any study classified as an RCT, with two or more experimental arms that evaluated any restorative treatment on primary teeth. The registries that did not result in any publications were excluded. Our search strategy identified 294 registries and 31 publications were included for data extraction. We have also calculated the median of journals' impact factors. Chi-square test was performed with 5% significance level. Our results found that the prevalence of SOR was identified in 19 papers (61.3%). The most common reason was a discrepancy in the primary outcome timeframe (48.4%). Twenty-six (83.9%) of the trials were registered retrospectively. Also, a discrepancy in sponsorship was identified in 22 (71%) of the trials. SOR was associated with the discrepancy in the follow-up period (p=0.003), with no association with registration timing (p=0.948). The impact factor of the journals ranged from 0.213 - 5.79. We found SOR in 9 (29%) of the six high impact factor journals and 10 (32.3%) of the eight with low impact.
Consequently, we emphasized the importance of transparency in RCT focusing on restorative care in children, including properly prospective registration and declaration of any discrepancy in the final manuscript, helping to avoid spin in science.
(Apoio: CAPES N° 001)
HA007 - Hatton
Área: 4 - Odontopediatria
Apresentação: 10/09 (Sexta-feira) - Horário: 10h30 - 12h00 - Sala: 7
Probiotic: in vivo evaluation of the effect on progression of periapical lesions, through microscopic analysis
Araujo LDC, Silva RAB, Messora MR, Furlaneto FAC, Salvador SLS, Nelson-Filho P, Pucinelli CM, Silva LAB
UNIVERSIDADE DE SÃO PAULO - RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse
The aim of the present study was to evaluate the effect of the probiotic Bifidobacterium animalis subsp. lactis (B. lacti) HN019 in vivo, as an irrigation solution for root canals in rats' teeth with induced periapical lesion. The animals were divided in four groups, as follows: group I: healthy teeth; group II: periapical lesion; group III: 2.5% sodium hypochlorite; group IV: probiotic, in 7, 21, 42 days. The probiotic irrigating solution was formulated by adding the probiotic at 2.7x 109 CFU in water medium and 2.0% of carboxymethylcellulose. The root canals were irrigated on day 0 and days 3, 7, 14, 21, 28 and 35 days, after periapical lesion induction. The mandible samples were stained in HE and analyzed under optical microscopy to describe the characteristics of the pulp tissue and the apical and periapical regions, in parallel with the morphometric analysis of the extension of the lesion area, osteoclast marking (TRAP), immunohistochemistry to identify markers of the osteoclastogenesis (RANK, RANK-L and OPG) and microbiological analysis using the Brown & Brenn technique. The data were analyzed using software R, version 4.0.0, applying SAS 9.4 and Graph Pad Prism 5, with a significance level of 5%. The histopathological, immunohistochemical and microbiological analysis parameters in probiotic groups were significantly better when compared to groups without periapical lesion and 2.5% sodium hypochlorite (p<0.05).
Therefore, probiotic demonstrated a therapeutic potential on the progression of periapical injuries.
(Apoio: FAPESP N° 2018/22038-3)
HA015 - Hatton
Área: 7 - Imaginologia
Apresentação: 10/09 (Sexta-feira) - Horário: 14h00 - 15h30 - Sala: 7
Using point coordinates to measure root canal curvature in periapical radiograph and CBCT images using free software
Gialain IO, Yanaguizawa WH, Fontana CE, Caldeira CL, Cavalcanti MGP
Ciências Odontológicas Integradas - UNIVERSIDADE DE CUIABÁ
Conflito de interesse: Não há conflito de interesse
This study proposed to demonstrate a technique to calculate root canal curvature on 2D and 3D images using point coordinate, named Point Method (PM), and further compare these methods to the most used method (Schneider). The total sample comprised 120 teeth (30 upper lateral incisors, 30 lower premolars, 30 upper molars, and 30 lower molars), with no radiopaque material on the root canal or pulp chamber. Each tooth underwent periapical radiograph and CBCT exams, and the curvature was analyzed by three different methods - Schneider, 2D PM, and 3D PM. Schneider and 2D PM were performed on periapical radiograph and 3D PM on CBCT. Point Method analysis was made placing points on the canal lumen with a vertical distance of 1mm among points. Using open-source software, the coordinates for each point were transferred into a spreadsheet with analytical geometry formulas. A repeated measures test was made to compare angular values among the methods and an independent measures test was performed to compare the dental groups and the root thirds. The mean curvature angle values found using 3D PM (89.01°) were significantly higher when compared to 2D PM (56.72°) and Schneider (34.62°) for all dental groups. Multirooted teeth had a greater mean curvature angle than single-rooted and the apical third also presented the most curved root canals amongst the root thirds.
The results presented herein show that 3D curvature was higher when compared with 2D analysis. Higher curvature angle values were found at the apical third and on multirooted teeth.
(Apoio: CAPES N° 1570958)
HA016 - Hatton
Área: 8 - Periodontia
Apresentação: 10/09 (Sexta-feira) - Horário: 16h00 - 17h30 - Sala: 7
Periodontal Collapse and Incipient Vascular Abnormalities forming a cluster at the end of 2nd decade of life: a population-based study
Costa CM, Costa SA, Souza SFC, Alves CMC, Thomaz EBAF, Silva AAM, Ribeiro CCC
Programa de Pós-graduação Em Odontologia - UNIVERSIDADE FEDERAL DO MARANHÃO
Conflito de interesse: Não há conflito de interesse
Periodontitis and cardiovascular disease continuums are associated with each other in late adulthood; we hypothesize this cluster may start as early as adolescence. Thus, we explored the association between initial periodontal collapse and incipient vascular abnormalities at the end of 2nd decade of life. Population-based sample from RPS Birth Cohort Consortium, follow-up at 18-19 years, São Luís (n=2515). The theoretical model explored the complex structure between Initial Periodontitis and the Cardiovascular Risk, adjusted to socioeconomic status, smoking, alcohol, and obesity, through Structural Equation Modelling. The Cardiovascular Risk was a latent variable representing the shared variance of carotid-femoral pulse wave velocity, systolic and diastolic blood pressures. Initial Periodontitis representing the shared variance of visible plaque index, bleeding on probing, probing depth ≥4mm, and clinical attachment loss ≥3mm. Socioeconomic Status reduced the Initial Periodontitis (Standardized Coefficient -SC: -0.132; p<0.001) and the Cardiovascular Risk values (SC: 0.069; p= 0.032); Obesity increased Initial Periodontitis (SC: 0.111; p=0.001) and Cardiovascular Risk values (SC: 0.390; p=<0.001). The Cardiovascular Risk and Initial Periodontitis associations were bidirectional ones (SC= 0.079; p=0.032).
The initial periodontal collapse and incipient vascular abnormalities in young formed a cluster. The attempt to reduce the diseases continuums should begin much early and direct to their common risk factors.
(Apoio: CNPq)
HA018 - Hatton
Área: 8 - Periodontia
Apresentação: 10/09 (Sexta-feira) - Horário: 16h00 - 17h30 - Sala: 7
Association between poor oral health and adverse COVID-19 outcomes in hospitalized patients
Costa CA, Vilela ACS, Oliveira SA, Leles CR, Costa NL
Periodontia - UNIVERSIDADE FEDERAL DE GOIÁS
Conflito de interesse: Não há conflito de interesse
This study aimed to assess the dental and periodontal status of hospitalized COVID-19 patients and their association with the incidence of adverse COVID-19 outcomes. 128 hospitalized patients with COVID-19 diagnosis were included. DMFT index, periodontal status, and tooth loss patterns (Eichner index) were assessed by means of in-hospital clinical examination. Associations between oral health measures, the severity of COVID-19 symptoms, and hospitalization endpoints were tested using chi-square test and prevalence ratio (PR) estimation using Generalized Linear Model (GLM) with log-Poisson regression with robust error variances. Oral health-related variables, comorbidities, and patient's age were included in the regression models using a block-wise selection of predictors. Poor oral health conditions were highly prevalent and associated with critical COVID-19 symptoms, higher risk for admission in the intensive care unit, and mortality. Periodontitis was significantly associated with ICU admission (PR=1.44; p=0.017), critical symptoms (PR=2.56; p=0.002), and risk of death (PR=2.02; p=0.028) when adjusted for age and comorbidities. Eichner index (B and C) was associated with ICU admission, probably affected by the relationship between advanced age and tooth loss.
The positive association between deleterious oral health-related conditions, especially periodontitis, and serious COVID-19 outcomes suggest that dental and periodontal status may play a role in the prognosis of hospitalized COVID-19 patients.
(Apoio: FAPEG N° #CVD2020051000009)
HA019 - Hatton
Área: 10 - Implantodontia básica e biomateriais
Apresentação: 10/09 (Sexta-feira) - Horário: 16h00 - 17h30 - Sala: 7
Tailoring Cu-loaded electrospun membrane with antibacterial ability for guided bone regeneration
Cordeiro JM, Avila ED, Yang F, Beucken JD, Barão VAR
Odontologia - CENTRO UNIVERSITÁRIO DAS FACULDADES ASSOCIADAS DE ENSINO
Conflito de interesse: Não há conflito de interesse
We tailored copper (Cu)-loaded electrospun membranes for guided bone regeneration (GBR) targeting the stimulation of innate cells active in bone growth and the prevention of treatment-related infections. Functional GBR membranes were produced via an electrospinning set-up using a silk-based solution in association with polyethylene oxide (Silk:PEO - control). Experimental groups were loaded with Cu oxide (CuO 1%, CuO 0.5%, CuO 0.1%, and CuO 0.05%). The morphological, structural, chemical and mechanical properties of membranes were evaluated. Direct and indirect in vitro cytocompatibility experiments were performed with human bone mesenchymal stem cells and human umbilical vein endothelial cells. The antibacterial potential of membranes was tested with Staphylococcus aureus biofilm (24 h). One-way ANOVA and Tukey's HSD test were used for multiple comparisons (p<0.05). CuO was successfully incorporated into membranes as clusters without affecting its mechanical resistance. Cu ions were released from the nanofibers over 1 week, being expressively greater in an acidic condition. Increased Cu concentration produced membranes with thinner nanofiber, greater porosity, and stronger antimicrobial effect (p<0.01). CuO 0.1% and CuO 0.05% were able to support and stimulate cells adhesion and proliferation (p<0.05), as well as angiogenesis.
The tailored functional Cu-loaded GBR membrane with combined cell stimulatory behavior and antibacterial capacity may be a valuable strategy to diminish the risk of infections and improve the regeneration process.
(Apoio: FAPs - Fapesp N° 2018/14117-0 | CNPq N° 304853/2018-6 | CAPES N° Finance Code 001)
HA020 - Hatton
Área: 10 - Implantodontia básica e biomateriais
Apresentação: 10/09 (Sexta-feira) - Horário: 16h00 - 17h30 - Sala: 7
Titanium with nanotopography inhibits osteoclast-induced disruption of osteoblast differentiation by regulating histone methylation
Bighetti-Trevisan RL, Almeida LO, Gordon J, Tye C, Lian JB, Stein GS, Rosa AL, Beloti MM
Curso de Odontologia - UNIVERSIDADE DE RIBEIRÃO PRETO
Conflito de interesse: Não há conflito de interesse
The aim of this study was to evaluate the regulation of the osteoclast/osteoblast crosstalk by titanium with nanotopography (Ti Nano) and the mechanism involved in this process. Osteoblasts were cultured on Ti Nano and Ti Machined and osteoclasts into inserts in a co-culture model for 48 h, and non-cocultured osteoblasts were used as control. The RNAseq (DESeq2: FC>1.7; p≤0.05) detected that osteoclasts downregulated the expression of genes related to osteogenesis and upregulated genes related to histone modification and chromatin organization in osteoblasts grown on both surfaces. Osteoclasts also inhibited (p≤0.05) the gene expression of Runx2, Dlx5, Alpl, Ibsp, Bglap and Opg and protein expression of RUNX2 and ALPL, and such effect was reduced by Ti Nano. Regarding the mechanism, an increase in H3K9me2, H3K27me3 and EZH2 protein expression was observed on both Ti surfaces under coculture conditions (p≤0.05). ChIP assay revealed accumulation of H3K9me2 repressing the promoter region of Alpl and H3K27me3 repressing Runx2, Alpl, Ibsp and Opg in osteoblasts in the presence of osteoclasts, which were attenuated by Ti Nano. Immunofluorescence corroborated the ChIP assay, exhibiting less RUNX2 and ALPL and more H3K27me3 staining in osteoblasts under coculture conditions in a less pronounced way on Ti Nano.
In conclusion, Ti Nano may favor the clinical outcomes of implant osseointegration, since this nanotopography inhibited the osteoclast-induced disruption of osteoblast differentiation by reducing the accumulation of H3K9 and H3K27 methylated histones.
(Apoio: FAPESP N° 2017/23888-8 | FAPESP N° 2018/17356-6 | CNPq N° 303464/2016-0)